MICROSCOPE TIME-SERIES PLAN AND PILOT RECORD
Yenra | Updated October 9, 2026
Guide: https://yenra.com/real-time-microscope/

Plan with the responsible laboratory and imaging specialist. This is an
acquisition worksheet, not a specimen-preparation or culture protocol.
Keep it with original data, timestamps and acquisition metadata.

Question / specimen ID / session date / operator:
Fastest change to recognize and basis for estimate:
Field / objective / contrast method / calibration:
Validated specimen conditions and responsible protocol:
Preview rate (frames/s):
Exposure (ms) or scan timing per channel:
Planned start-to-start interval (s):
Duration (s) / are both endpoints included?:
Channels / planes / positions per time point:
Measured time to acquire and save a full time point (s):
Focus-control method / reference and offset:
Light intensity, shuttering, controls and pilot endpoints:

DATA BUDGET
Time points = duration / interval + 1 only for exactly divisible duration
with both endpoints included. Check the software's counting convention.
Total images = time points x channels x planes x positions.
Uncompressed grayscale bytes/image = width x height x stored bytes/pixel.
Allow additional space for metadata, previews, processing and backup copies.
Actual saved-file test / output format / storage destination:
Budget / free capacity / backup plan:

ILLUSTRATIVE EXAMPLE
0 through 1800 s inclusive, every 10 s: 181 time points.
181 x 2 channels x 3 planes x 1 position = 1086 images.
1024 x 1024 x 2 bytes (16-bit grayscale) = 2097152 bytes/image.
1086 x 2097152 = 2277507072 bytes, about 2.28 GB or 2.12 GiB.
One selected image/time point exported at 20 frames/s:
181 / 20 = 9.05 s of playback, representing 30 min first-to-last observation.

PILOT CHECKS
Reopen saved data: dimensions, channels and plane order correct?:
Timestamp interval measured / missing frames / delays:
Focus at beginning, middle and end:
Specimen condition / controls / light-related changes:
Stationary landmarks / drift / lost fields:
Change made after pilot and reason:

FINAL RECORD
Original-data path / timestamps / settings:
Pauses, interventions, failures:
Processing (registration, cropping, projections, intensity adjustments):
Movie rate / displayed elapsed time / acquisition interval / scale:
Interpretation limits and follow-up: