PHOTOMICROGRAPH CAPTURE AND CALIBRATION RECORD
Guide: https://yenra.com/microscopes/photography.html
Keep the original capture and this record together. Use one record per
configuration; repeat spatial calibration after changes to image sampling.
Date / specimen ID / description:
Microscope / objective / adapter / camera:
Illumination method and settings:
Exposure / gain or ISO / white balance:
Capture pixel dimensions / binning / optical or digital zoom:
Original image filename:
Calibration slide identity and verified division size:
Calibration image filename (same acquisition settings):
Known distance (micrometers):
Measured pixel distance:
Scale = known distance / pixel distance (micrometers per pixel):
Desired scale-bar length (micrometers):
Bar pixels = bar length / scale:
Worked example ONLY: 100 um / 400 px = 0.25 um/px; 50 um bar = 200 px.
Use your own measured calibration, not the example numbers.
Processing and export history (crop, resize, color, stack, other):
Final output filename / calibration verified after export:
Caption: specimen, method, objective, calibrated scale, consequential edits.