PROTEIN CRYSTALLIZATION — SCREEN AND HIT RECORD
Version: 2026-09-11
Guide: https://yenra.com/crystallization/screening-chip.html

Purpose: preserve conditions and observations so a promising trial can be
repeated and followed toward diffraction. Use validated laboratory methods
and device documentation for experimental recipes and operations.

Operator / date / project:
Sample identity / construct / ligand / preparation batch:
Concentration and units / preparation record:
Screen name / version / complete condition-definition file:
Chip type / identifier / trial map / loading method:
Temperature / loading time / observation schedule:
Sample use, losses and remaining material:
Sealing / filling checks / bubbles / evaporation / setup deviations:
Trials affected by setup problems (keep separate from chemical outcomes):

OBSERVATIONS — copy for each trial and time point
Trial ID and exact recoverable composition (concentrations and units):
Time since loading / image file and scale:
Observed category: clear / precipitate / phase separation /
crystalline-looking object / other:
Description and changes since prior observation:
Interpretation, with uncertainty:
Confirmation planned / method / result:

Fictional example: B7 shows fine precipitate at 24 hours and a small faceted
object at 72 hours. Save both images and times. Record an apparent crystalline
hit awaiting identification; recover the exact B7 composition and repeat.
A faceted object alone does not establish that it is a protein crystal.

FOLLOW-UP
Reference condition and independent repeat:
Variables deliberately changed / local range / controls:
Sample batch or setup differences from the initial screen:
Success criterion (reproducible growth, morphology, diffraction, etc.):
Access to harvesting, transfer or compatible on-chip diffraction:
Identification evidence / diffraction result / structural-data record:
Ambiguous or failed trials and what they imply for the next experiment:
Next decision / responsible person / linked records:

Retain clear, failed and ambiguous trials as well as hits. Keep observation,
identification and diffraction quality as separate recorded milestones.