CELL-IMAGING REVIEW WORKSHEET
Updated September 16, 2026 | Yenra
Guide: https://yenra.com/biomedical-testing/

Purpose: record the information needed to interpret a cell-imaging result.
Complete one sheet per biological comparison. Mark unknown information as
unknown and identify who or what document can resolve it. This is a research
review aid; use the facility's validated procedures for laboratory work.

1. QUESTION AND DESIGN
Biological question and expected measurement:
Specimen/cell type and relevant identifiers:
Independent biological unit and number of independent replicates:
Conditions, time points, positive/negative controls:
Plate layout, processing batches and acquisition order:

2. IMAGES AND ANALYSIS
Original-image location and acquisition metadata:
Channels, exposure, objective and pixel scale:
Software/version, pipeline/settings, model version if used:
Object definition (nucleus, cell, other) and segmentation checks:
Representative overlays from each condition reviewed:
Background, focus, saturation, illumination and cell-loss checks:
Prespecified exclusions and actual exclusions with reasons:

3. DENOMINATORS AND RESULTS
Accepted total cell count per well/sample:
Positive cell count and definition of positive:
Positive fraction (%) = 100 x positive count / accepted total count.
If total count is zero, the fraction is undefined; do not report zero percent.
Summaries across independent replicates, uncertainty and exclusions:
Effect size and sensitivity to plausible analysis settings:

FICTIONAL EXAMPLE (two wells; not sufficient biological replication)
Control: 200 positive / 1000 accepted cells = 20%.
Treated: 120 positive / 400 accepted cells = 30%.
Difference: 10 percentage points; positive-cell count falls by 80 cells.
Check cell loss, viability-related evidence and segmentation before assigning
a biological explanation. Compare independent replicates next.

4. CONCLUSION AND NEXT STEP
What the measurement establishes:
What remains uncertain:
Independent confirmation or other follow-up needed:

PRIMARY METHODS REFERENCES
https://www.ncbi.nlm.nih.gov/books/NBK100913/
https://www.ncbi.nlm.nih.gov/books/NBK126174/
https://tutorials.cellprofiler.org/